Pullulanase is a specific kind of glucanase , an amylolytic exoenzyme , that degrades pullulan . It is produced as an extracellular, cell surface anchored lipoprotein by Gram negative bacteria of the genus Klebsiella . Type I pullulanases specifically attack 1,6 linkages, while type II pullulanases are also able to hydrolysis hydrolyse 1,4 linkages. It is also produced by some other bacteria and archaea . Pullulanase is used as a processing aid in grain processing biotechnology production of ethanol and sweeteners . Pullulanase EC number 3.2.1.41 is also known as pullulan 6 glucanohydrolase Debranching enzyme . Its substrate, pullulan, is regarded as a chain of maltotriose units linked by alpha 1,6 glycosidic bond s. Pullulanase will hydrolytically cleave pullulan alpha glucan polysaccharides . External links MeshName pullulanase Category Enzymes Bacteria stub Enzyme stub de Pullulanase id Pululanase ... more details
chembox verifiedrevid 451781241 ImageFile Pullulan.png ImageSize IUPACName OtherNames E1204 Section1 Chembox Identifiers CASNo Ref cascite correct CAS CASNo 9057 02 7 EINECS 232 945 1 PubChem UNII Ref fdacite correct FDA UNII 8ZQ0AYU1TT SMILES Section2 Chembox Properties Formula C sub 6 sub H sub 12 sub O sub 5 sub sub n sub MolarMass Appearance White powder Density MeltingPt BoilingPt Solubility Soluble Section3 Chembox Hazards MainHazards FlashPt Autoignition Pullulan is a polysaccharide polymer consisting of maltotriose units, also known as 1,4 1,6 glucan . Three glucose units in maltotriose are connected by an 1,4 glycosidic bond , whereas consecutive maltotriose units are connected to each other by an 1,6 glycosidic bond . Pullulan is produced from starch by the fungus Aureobasidium pullulans . As an edible, mostly tasteless polymer, the chief commercial use of pullulan is in the manufacture of edible films that are used in various breath freshener or oral hygiene products such as Listerine Cool Mint. As a food additive , it is known by the E number E1204. See also Pullulanase References http ift.confex.com ift 2001 techprogram paper 8310.htm Characteristics of pullulan based edible films abstract http www.hayashibara.co.jp hbl product spec pu101.html Pullulan MeshName pullulan http www.sigmaaldrich.com Area of Interest Biochemicals Enzyme Explorer Key Resources Carbohydrate Analysis Carbohydrate AnalysisIII.html Pullulanase Pullulan chemical diagram Category Biotechnology products Category Food additives Category Polysaccharides Category Packaging materials biotech stub ar de Pullulan es Pululano fr Pullulane nl Pullulan ja ... more details
wiktionary Glucanases are enzyme s that break down a glucan , a polysaccharide made of several glucose sub units. As they perform hydrolysis of the glucosidic bond, they are hydrolase s. glucanases 1,4 glucanase , an enzyme that breaks down 1,4 glucan s 1,6 glucanase , an enzyme that breaks down 1,6 glucan s Pullulanase , a specific kind of glucanase that degrades pullulan glucanases 1,3 glucanase , an enzyme that breaks down 1,3 glucan s such as callose or curdlan 1,6 glucanase , an enzyme that breaks down 1,6 glucan s Cellulase , an enzyme that perform the hydrolysis of 1,4 beta D glucosidic linkages in cellulose , lichenin and cereal beta D glucan s. Xyloglucan specific endo beta 1,4 glucanase Xyloglucan specific exo beta 1,4 glucanase Occurrences Glucanases can be produced by Neocallimastigomycota , a phylum of anaerobic fungi found in the digestive tracts of herbivores. See also Glycoside hydrolase s, a family of enzyme that cut a glycoside from a non glycosidic molecule Glycoside hydrolase family 5 Glycoside hydrolase family 16 Glycoside hydrolase family 17 Category Hydrolases enzyme stub ... more details
Glucosidases are glycoside hydrolase enzymes categorized under the Enzyme Commission number EC number 3.2.1. Function glucosidases are enzymes involved in breaking down complex carbohydrates such as starch and glycogen They catalyze the cleavage of individual glucosyl residues from various glycoconjugates including alpha or beta linked polymers of glucose. Members Different sources include different members in this class. Members marked with a are considered by Medical Subject Headings MeSH to be glucosidases. class wikitable Name EC Description Amylase Amylase Amylase EC number 3.2.1.1 is a digestion digestive enzyme in mammal s Amylase Amylase Amylase EC number 3.2.1.2 is a plant enzyme to break down starch Amylase Amylase Amylase EC number 3.2.1.3 is a digestive enzyme Cellulase EC number 3.2.1.4 breaks down cellulose from plant material Sucrase isomaltase EC number 3.2.1.10 Acid glucosidase EC number 3.2.1.20 is associated with Glycogen storage disease type II Beta glucosidase EC number 3.2.1.21 is associated with gaucher s disease Lactase EC number 3.2.1.23 one member of the Beta galactosidase galactosidase family, breaks down milk sugars, and its absence in adult hood causes lactose intolerance glycogen debranching enzyme Debranching enzyme EC number 3.2.1.33 Pullulanase EC number 3.2.1.41 has been used as a detergent Clinical significance They are targeted by alpha glucosidase inhibitor s such as acarbose and miglitol to control diabetes mellitus type 2 . See also DNA glycosylases Mucopolysaccharidoses External links MeshName Glucosidases Glycoside hydrolases Category Enzymes Category Hydrolases Category Carbohydrates Category EC 3.2 ... more details
it with both alpha amylase and pullulanase in order to analyze it, found that some starch remained ... pre processed with the enzyme pullulanase. That source had the second highest alpha amylase resistant ... more details
substrates ie. isoamylase, pullulanase and branching enzyme. Isoamylase hydrolyses 1,6 alpha D glucosidic ... in the formation of 1,6 glucosidic linkages of glycogen and pullulanase is a starch debranching ... more details
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