- Electropherogram
File Chromatogram.jpg thumb upright 2 Screen shot of a chromatogram inside the program Sequencher An electropherogram is a plot of results from an analysis done by electrophoresis automatic sequencing. Electropherograms may be used for deriving results from genealogical DNA test ing parental testing paternity test ing DNA sequencing Genetic fingerprinting An electropherogram shows a sequence of data that is produced by an automated DNA sequencing machine. ref http dna glossary.dnawiz.com Electropherogram.html DNA Glossary ref References references See also DNA electrophoresis Electrophoretogram External links http condor.genoma.cenargen.embrapa.br phph PHPH web based tool for electropherogram quality analysis http journalsip.astm.org jofs PAGES 4390.htm Systematic differences in electropherogram peak heights reported by different versions of the GeneScan Software http dna.reinyday.com 464 DYS464 Electropherogram Interpretation Discrepancy with images http bscs.org pages curriculumdevelopment onco images sprotocol normal.gif electropherogram image Category Laboratory techniques Category DNA Category Biometrics Category Applied genetics Category Genetic genealogy es Electroferograma de Elektropherogramm ... more details
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- File:Electropherogram trace.jpg
Summary DNA electropherogram trace. Licensing PD self date May 2007 duplicate file info otheraddress Image Electropherogram Trace.jpg othertext big Summary big Example of a DNA electropherogram trace big Licensing big Template PD self end duplicate file info template Copy to Wikimedia Commons bot Fbot priority true ... more details
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- Electrophoretogram
An electrophoretogram is the result of an electrophoresis , which gives the movement of charged particles over time in a Gel electrophoresis gel , paper or another medium. References references See also DNA electrophoresis , an analytical technique used to separate DNA fragments by size Electropherogram , a plot of fluorescence units over time Category Laboratory techniques Category Electrophoresis ... more details
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- Finisher
Finisher may refer to In the field of genome sequencing , a finisher is one who analyzes an electropherogram , a graphical representation of data received from a sequencing machine and yields one read, in comparison to the consensus, a sequence that has been generated from the alignment of multiple reads, presented by the computer program s Phred quality score Phred and Phrap , to determine if the consensus accurately reflects the nucleotide sequence when the data is incomplete or inadequate, the finisher calls more reactions with appropriate chemistry in order to gather more data. In computing, a printer computing printer finisher performs post printing actions, such as stapling, hole punching, folding, or collating. In cricket , the term finisher can also refer to the name given to the role played by a Not out not out batsman that results in his her team winning the match. Textile manufacturing Finishing processing of textiles Finisher textiles , the processes after weaving such as bleaching, dyeing and printing Finisher wrestling , a move in professional wrestling disamb de Finisher ... more details
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- Terminal restriction fragment length polymorphism
profiling is a graph called electropherogram which is an intensity plot representation of an electrophoresis experiment gel or capillary . In an electropherogram the X axis marks the sizes of the fragments ... on an electrophoresis gel as a band appears as a peak on the electropherogram whose integral is its ... in a population might give a single peak on the electropherogram due to the presence of a restriction .... Interpretation of data The data resulting from the electropherogram is normally interpreted in one ... computer applications attempt to relate the peaks in an electropherogram to specific bacteria in a database ... and several minor peaks which appear are irreproducible the overall shape of the electropherogram ... in one peak only on the electropherogram and will be indistinguishable. Indeed, when T RFLP ... more details
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- QPNC-PAGE
of the gel may affect the peak elution times of separated metalloproteins in the electropherogram ... Figure Electropherogram . In order to obtain a fully polymerized gel for a PAGE run, the polyacrylamide ... ref Reproducibility and recovery File Preparative Electroph.TIF thumb 315px Electropherogram ... reproducibly in the same fraction after a PAGE run see Figure Electropherogram . A shifting peak in the respective electropherogram may indicate that a denatured metalloprotein is available in the original ... more details
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- Gel electrophoresis of nucleic acids
electrophoresis results are typically displayed in a trace view called an electropherogram . Factors ... more details
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- List of genetic genealogy topics
genetic genealogy This is a list of genetic genealogy topics . Important concepts Genetic genealogy Genealogical DNA test Human mitochondrial DNA haplogroups Human Y chromosome DNA haplogroups Allele Allele frequency Electropherogram Genetic recombination Haplogroup Haplotype haplogroup Most recent common ancestor Short tandem repeat STR Single nucleotide polymorphism SNP Y STR Y chromosome short tandem repeat Related fields Archaeogenetics Genealogy Genetics Genetic fingerprinting DNA sequencing Population genetics Molecular genetics Patrilineal relationships Patrilineality XY sex determination system Y chromosomal Adam Y chromosomal Aaron Adam s Curse Paternal mtDNA transmission RecLOH Matrilineal relationships Matrilineality Mitochondrion Mitochondrial DNA Human mitochondrial genetics Mitochondrial Eve X chromosome Biogeography, ethnicity and migration Human migration Population genetics Multiregional hypothesis Single origin hypothesis Projects Human Genome Project International HapMap Project Molecular Genealogy Research Project Surname DNA project The Genographic Project Lists List of Y STR public databases List of Y chromosome databases List of DNA tested mummies List of DNA tested historical figures List of genetic results derived from historical figures Y chromosome haplogroups by populations See also List of genetics related topics human genetics Category Genetic genealogy Category Biology lists Genetic genealogy Category Outlines Genetic genealogy topics Category Outlines ... more details
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- Microsatellite instability
Cleanup date April 2008 Microsatellite genetics Microsatellites are repeated sequences of DNA. Although the length of these microsatellites is highly variable from person to person, each individual has microsatellites of a set length. These repeated sequences are common, and normal. The most common microsatellite in humans is a dinucleotide repeat of CA, which occurs tens of thousands of times across the genome. Image Microsatellite Instability in GeneMarker.jpg thumb 400px Example of Microsatellite Instability in a DNA Electropherogram Trace In cells with mutations in DNA repair genes, however, some of these sequences accumulate errors and become longer or shorter. The appearance of abnormally long or short microsatellites in an individual s DNA is referred to as microsatellite instability. Microsatellite instability MSI is a condition manifested by damaged DNA due to defects in the normal DNA repair process. ref MeshName Microsatellite Instability ref Sections of DNA called Microsatellite genetics microsatellite s, which consist of a DNA sequence sequence of repeating units of 1 6 base pair s in length, become unstable and can shorten or lengthen. Microsatellites are also known as simple sequence repeats SSRs . Clinical significance MSI is a key factor in several cancers including colorectal, endometrial, ovarian and gastric cancers. Colorectal cancer studies have demonstrated two mechanisms for MSI occurrence. The first is in hereditary nonpolyposis colorectal cancer HNPCC or Lynch Syndrome, where an inherited mutation in a mismatch repair gene causes a microsatellite repeat replication error to go unfixed. Most cases result in changes in the lengths of dinucleotide repeats of the nucleobase s cytosine and adenine sequence CACACACACA... . ref cite pmid 10321739 ref The replication error results in a frameshift mutation that inactivates or alters major tumor suppressor genes key genes in the regulation of the cell cycle and, ultimately, the prevention of cancer. T ... more details
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- Label-free HPCE
Label free HPCE High Performance Capillary Electrophoresis is used in the life science industries for the analysis of biomolecules, principally in the detection and quantification of protein , DNA and RNA . Process Label free HPCE systems utilise Label free intrinsic imaging technology LFII and the data analysis of DNA and proteins in such systems are enabled by Generalized Separation Transform GST and Equiphase Vertexing EV algorithms. ref http www.selectscience.net application notes deltadot analysis of immunoglobulin g igg using deltadots peregrine high performance capillary electrophoresis hpce with label free intrinsic imaging lfii ?artID 13016 ref LFII eliminates the need for labels commonly required in capillary electrophoresis CE systems. GST is a method of combining the data from multiple pixel diode arrays in a way that preserves the peak shape information of the electropherograms. EV is a technique that uses a space time constraint to significantly increase the signal to noise ratio , to improve Sensor resolution resolution and to allow associations of a given band in the electrophoretic window with a given injection. In EV the electropherogram s are first analyzed to find local peaks that are then used to determine the origin of the bands through vertexing and then to produce a significantly enhanced signal output. Advantages Multipoint detection and label free photonic imaging have mathematical and physicochemical consequences that provide important advantages. First the geometric method of analysis makes it possible to use powerful signal processing algorithms that are computationally highly efficient and can run in real time on a standard PC. Secondly, the ability to use powerful signal processing algorithms allows the use of very low signal levels which permits the use of photonic imaging rather than label detection. Thirdly, photonic imaging has the advantage over the use of labels that the strength of the signal depends on the absorption of light b ... more details
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- Amplified fragment length polymorphism
Image Electropherogram trace.jpg thumb 300px Example of AFLP Data from a Capillary Electrophoresis Instrument Amplified Fragment Length Polymorphism PCR or AFLP PCR or just AFLP is a polymerase chain reaction PCR based tool used in genetics research, DNA fingerprint ing, and in the practice of genetic engineering . Developed in the early 1990s by Keygene, ref http www.keygene.com ref AFLP uses restriction enzymes to digest Genome genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction fragment s. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments as described in detail below . The amplified fragments are visualized on denaturing polyacrylamide electrophoresis gels either through autoradiography or fluorescence methodologies. AFLP PCR is a highly sensitive method for detecting Polymorphism biology polymorphism s in DNA . The technique was originally described by Vos and Zabeau in 1993. ref Zabeau, M and P. Vos. 1993. Selective restriction fragment amplification a general method for DNA fingerprinting. European Patent Office, publication 0 534 858 A1, bulletin 93 13. ref ref cite journal author Vos P, Hogers R, Bleeker M, et al. title AFLP a new technique for DNA fingerprinting journal Nucleic Acids Res. volume 23 issue 21 pages 4407 14 year 1995 month November pmid 7501463 pmc 307397 url http nar.oxfordjournals.org cgi pmidlookup?view long&pmid 7501463 doi 10.1093 nar 23.21.4407 ref In detail, the procedure of this technique is divided into three steps http www.keygene.com keygene techs apps FLASH3.php Digestion of total cellular DNA with one or more restriction enzyme s and ligation of restriction half site specific adaptors to all restriction fragments. Selective amplification of some of these fragments with two PCR primers that have corresponding ... more details
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- Capillary electrophoresis
device such as an integrator or computer . The data is then displayed as an electropherogram ... with different migration times in an electropherogram. ref name Baker Skoog, D.A. Holler, F.J. ... more details
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- Nucleic acid sequence
bind to the sense strand. Sequence determination Image DNA sequence.svg right thumb 268px Electropherogram ... more details
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- Genetic genealogy
Allele Allele frequency Electropherogram Family name Genealogical DNA test Genealogy Genetic recombination ... more details
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- Genealogical DNA test
genealogy topics Div col cols 2 Archaeogenetics Electropherogram Family name Patrilineal surname FamilyTreeDNA ... more details
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