- DAPI
chembox verifiedrevid 443556310 ImageFile DAPI.svg ImageSize 200px ImageFile1 DAPI Space Fill.png ImageSize1 ... DAPI or 4 ,6 diamidino 2 phenylindole is a fluorescence fluorescent staining biology stain ... . DAPI can pass through an intact cell membrane therefore it can be used to stain both live and Fixation ... the effectiveness of the stain is lower. History DAPI was first synthesised in 1971 in the laboratory ... in 1975, the first recorded use of DAPI as a fluorescent DNA stain. ref name Kapuscinski1995 cite journal author Kapuscinski J title DAPI a DNA specific fluorescent probe journal Biotech Histochem volume ... when bound to DNA led to the rapid adoption of DAPI for fluorescent staining of DNA for fluorescence .... Use of DAPI as a DNA stain for flow cytometry was also demonstrated around this time. ref name Kapuscinski1995 Fluorescence properties When bound to double stranded DNA DAPI has an absorption maximum ... for fluorescence microscopy DAPI is excited with ultraviolet light and is detected through a blue cyan filter. The emission peak is fairly broad ref name DAPI Nucleic Acid Stain Invitrogen, http probes.invitrogen.com media pis mp01306.pdf DAPI Nucleic Acid Stain . accessed 2009 12 08. ref DAPI ... 500 nm when bound to RNA. ref Scott Prahl, http omlc.ogi.edu spectra PhotochemCAD html dapi H2O .html DAPI . accessed 2009 12 08. ref File 1D30 DNA DAPI.png 250px left thumb DAPI magenta bound to the minor groove of DNA green and blue . From PDB 1D30 . DAPI s blue emission is convenient for microscopists ... overlap between DAPI and green fluorescent molecules like fluorescein and green fluorescent protein ... precise image analysis is required. Outside of analytical fluorescence light microscopy DAPI is also ... mycoplasma or virus particles in the growth medium fluoresce once stained by DAPI making them easy to detect. Live cells and toxicity DAPI can be used for both fixed and live cell staining, though the concentration of DAPI needed for live cell staining is generally much higher than for fixed ... more details
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- Dapi, Yunlin
Dapi Township is a rural township in Yunlin County , Taiwan . It has a population total of 21,523 and an area of 44.9973 square kilometres. Transportation Empty section date July 2010 Attractions Empty section date July 2010 External links http www.tapi.gov.tw Dapi Government website Taiwan Yunlin divisions coord missing Taiwan Category Townships in Yunlin County taiwan geo stub zh min nan To pi hiong hak Thai ph hi ng ko ja vi i B , V n L m zh ... more details
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- File:SibeliusGrave.jpg
Summary Information Description The grave of Jean Sibelius at Ainola Source I User DaPi DaPi User talk DaPi talk created this work entirely by myself. Date 3 July 2008 Author User DaPi DaPi User talk DaPi talk other versions Licensing self cc by sa 3.0 GFDL migration redundant Copy to Wikimedia Commons bot Fbot priority true ... more details
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- File:Stomata.jpg
Summary Stomata in the epidermal surface of an Aribidopsis thaliana leaf. DAPI stain highlights the epidermal pavement cells as well as the nuclei in the stomata guard cells. Licensing GFDL self with disclaimers migration relicense Copy to Wikimedia Commons bot Fbot ... more details
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- Trypanocidal agent
A trypanocidal agent is an antiprotozoal agent that acts upon on Trypanosoma trypanosome parasites. ref MeshName Trypanocidal Agents ref Examples include ref MeshPharmaList 82014344 ref benzonidazole blasticidin S DAPI diminazene diminazene aceturate eflornithine ethidium isometamidium chloride lonidamine melaminylthioarsenate melarsoprol nifurtimox pentamidine posaconazole puromycin quinapyramine salicylhydroxamic acid suramin tetraphenylporphine sulfonate See also Trypanosomiasis vaccine References Reflist Antiprotozoal agent antiinfective drug stub Category Antiprotozoal agents ... more details
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- Provincial Highway 78 (Taiwan)
Provincial Highway No. 78 begins in Taixi, Yunlin , Taiwan , on Zhonghua Road Provincial Highway No. 17 and ends in Gukeng, Yunlin on National Highway No. 3 Taiwan National Highway No. 3 . Length The total length is 43.5 km. Major Cities Along the Route Douliu City Intersections with other Freeways and Expressways National Highway No. 1 Taiwan National Highway No. 1 at Yunlin SIC. in Dapi, Yunlin National Highway No. 3 Taiwan National Highway No. 3 at Gukeng SIC. in Gukeng, Yunlin Provincial Highway No. 61 Taiwan Provincial Highway No. 61 at Taixi IC. in Taixi, Yunlin Notes Completed in December 2004 . References http www.thb.gov.tw Freeways and Expressways in Taiwan Taiwan Provincial Highways Category Highways in Taiwan Taiwan road stub zh 78 ... more details
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- Chromatin bridge
Infobox disease Name Chromatin bridge Image Chromatin bridge stained with DAPI 2.tiff Alt Caption DAPI staining allows for visualization of deoxyribonucleic acid portions of the two daughter cells. The thin string like DNA connecting them is defined as a chromatin bridge. DiseasesDB ICD10 ICD9 ICDO OMIM MedlinePlus eMedicineSubj eMedicineTopic MeshID GeneReviewsID GeneReviewsName Chromatin bridge is a mitotic occurrence that forms when telomeres of sister chromatids fuse together and fail to completely segregate into their respective daughter cells. Because this event is most prevalent during anaphase , the term anaphase bridge is often used as a substitute. After the formation of individual daughter cells, the DNA bridge connecting homologous chromosomes remains fixed. As the daughter cells exit mitosis and re enter interphase , the chromatin bridge becomes known as an interphase bridge. These phenomenon are usually visualized using the laboratory techniques of staining and fluorescence microscopy . ref name chan cite journal author Chan KL, Hickson ID title New insights into the formation and resolution of ultra fine anaphase bridges journal Semin Cell Dev Biol. year 2011 volume issue url http www.sciencedirect.com science article pii S1084952111000875 ref ref name hoffelder cite journal author Hoffelder D, Luo L, Burke N, Watkins S, Gollin S, Saunders W title Resolution of anaphase ... n2 full 6600438a.html ref Chromatin bridges may serve as a marker of cancer activity. File DAPI and tubulin ... and DAPI staining were utilized. C. The same cells visualized using DAPI staining. Process of Formation ... File Chromatin bridge stained using DAPI 1.tiff thumb A chromatin bridge, visualized using DAPI staining ... of a cell. DAPI is a fluorophore that specifically binds to DNA and fluoresces blue. In addition ... bridges are easiest and most readily visible when observing chromosomes stained with DAPI. DNA ... cells using indirect immunofluorescence is time consuming. Even though DAPI staining is quick, neither ... more details
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- Sandal spike phytoplasma
DAPI stain. Current Science 74, 989 993. Thomas, S. and Balasundaran, M. 1999 . Detection of sandal ... more details
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- Yunlin County
Dapi, Yunlin Dapi Township Ta p i Dapi T a pi Thai ph Dongshi, Yunlin Dongshi Township ... more details
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- Binucleated cells
be made visible so that binucleation can be identified. DAPI is a dye that binds to DNA and fluoresces ... with DAPI, highlighting the nuclei. C Both photos superimposed. Cancer Binucleation occurs at a much ... more details
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- Hoechst stain
for another nucleic acid stain called DAPI . Key differences between Hoechst dyes and DAPI are Hoechst dyes are less toxic than DAPI, which ensures a higher viability of stained cells ref cite ... of Hoechst 33258 and DAPI binding to five A T 4 DNA sites with kinetic evidence for more than one high ... Bisbenzimide Carcinogen DAPI DNA binding protein Emission Excited state Excitation Fluorescence Flourescence ... more details
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- Multipolar spindles
spindles with chromosomes under DAPI fluorescent stain..tif thumb This cell shows chromosomal aberration due to multipolar spindle attachment during metaphase under DAPI staining. Research has ... more details
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- Kinetoplast
A kinetoplast is a disk shaped mass of circular DNA inside a large mitochondrion that contains many copies of the mitochondrial genome . ref cite journal author Shapiro TA, Englund PT title The structure and replication of kinetoplast DNA journal Annu. Rev. Microbiol. volume 49 pages 117 43 year 1995 pmid 8561456 doi 10.1146 annurev.mi.49.100195.001001 ref ref cite journal author Shlomai J title The structure and replication of kinetoplast DNA journal Curr. Mol. Med. volume 4 issue 6 pages 623 47 year 2004 pmid 15357213 doi 10.2174 1566524043360096 ref Kinetoplasts are only found in protozoa of the class Kinetoplastida . Kinetoplasts are usually adjacent to the organisms flagellum flagellar basal body leading to the thought that they are tightly bound to the cytoskeleton . Trypanosoma In Trypanosomes , a group of flagellated protozoans, the kinetoplast exists as a dense granule of DNA within the large mitochondrion. Trypanosoma brucei , the parasite which causes African trypanosomiasis African sleeping sickness , is an example of a trypanosome with a kinetoplast. Its kinetoplast is easily visible in samples stained with DAPI , a fluorescent DNA stain biology stain . The kinetoplast contains circular DNA in two forms minicircles and maxicircles. Maxicircles are between 20 and 40kb in size and there are a few dozen per kinetoplast. There are several thousand minicricles per kinetoplast and these are between 0.5 and 1kb in size. The maxicircles encode the typical protein products needed for the mitochondria which is encrypted. Herein lies the only known function of the minicircles producing guide RNA gRNA to decode this encrypted information. ref cite journal author Lukes J. et al title Kinetoplast DNA Network Evolution of an Improbable Structure journal Eukaryotic Cell volume 1 pages 495 502 year 2002 doi 10.1128 EC.1.4.495 502.2002 ref This unique mode of DNA replication may represent potential drug targets. ref Unique characteristics of the kinetoplast DNA replicati ... more details
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- Barr body
Image Sd4hi unten crop.jpg thumb Nucleus of a female amniotic fluid cell. Top Both X chromosome territories are detected by Fluorescence in situ hybridization FISH . Shown is a single optical section made with a Confocal laser scanning microscopy confocal microscope . Bottom Same nucleus stained with DAPI and recorded with a Charge coupled device CCD camera . The Barr body is indicated by the arrow, it identifies the inactive X Xi . Image BarrBodyBMC Biology2 21 Fig1clip293px.jpg thumb Left DAPI stained female human fibroblast with Barr body arrow . Right histone macroH2A1 staining. Arrow points to sex chromatin in DAPI stained cell nucleus, and to the corresponding sex chromatin site in the histone macroH2A1 staining. A Barr body named after discoverer Murray Barr ref Barr, M. L., Bertram, E. G., 1949 , A Morphological Distinction between Neurones of the Male and Female, and the Behaviour of the Nucleolar Satellite during Accelerated Nucleoprotein Synthesis. Nature journal Nature . 163 4148 676 7. ref is the inactive X chromosome in a female somatic cell, ref Lyon, M. F., 2003 , The Lyon and the LINE hypothesis. j.semcdb 14, 313 318. http dx.doi.org 10.1016 j.semcdb.2003.09.015 Abstract ref rendered inactive in a process called lyonization , in those species including humans in which sex is determined by the presence of the XY sex determination system Y or ZW sex determination system W chromosome rather than the Haplodiploid sex determination system diploidy of the X or Z. The Lyon hypothesis states that in cells with multiple X chromosome s, all but one are inactivated during mammalian embryogenesis . ref Lyon, M. F. 1961 , Gene Action in the X chromosome of the Mouse Mus musculus L. Nature journal Nature . 190 4773 372 3. DOI 10.1038 190372a0 PMID 13764598 http www.nature.com nature journal v190 n4773 abs 190372a0.html Abstract ref This happens early in embryo nic development at random in mammal s, ref name Full Text PDF Brown,C.J., Robinson,W.P., 1997 , XIST Exp ... more details
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- Tipifarnib
by this age. Staining was smooth muscle alpha actin green , lamins A C red and DAPI blue . Original ... more details
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- Trigona (genus)
r5bri Cite journal author Brito and Pompolo title C G patterns and fluorochrome staining with DAPI and CMA3 ... more details
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- Cell cycle analysis
widely cited today. Experimental procedure File 1D30 DNA DAPI.png 250px right thumb DAPI magenta bound ... D , DAPI and Hoechst 33342 . All of them have in common that they Intercalation chemistry intercalate ... more details
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- Q-FISH
to complimentary DNA. Wash slides to remove unbound PNAs and counterstain DNA DAPI or PI ... medium is then placed onto the cells. This medium generally contains DAPI a DNA counterstain and an antifade ... more details
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- Nucleoid
also be seen under a light microscope. The DNA intercalating stains DAPI and ethidium bromide are widely ... more details
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- Ninong Ering
Infobox Indian politician honorific prefix name Ninong Ering honorific suffix image imagesize alt caption constituency MP Arunachal East Lok Sabha constituency Arunachal East parliament majority term start 1 June 2009 term end predecessor successor birth date birth date and age 1959 03 01 df yes birth place Pasighat , East Siang , Arunachal Pradesh death date death place restingplace restingplacecoordinates birthname File ninong ering.jpg 200px nationality IND party Indian National Congress otherparty For additional political affiliations spouse Smt. Yarok Ering br Married on 07 March 1979 partner For those with a domestic partner and not married relations children 2 Sons and 2 Daughters residence Mirku Dapi Village br P.O. Pasighat br Distt. East Siang br Arunachal Pradesh br 92, South Avenue br New Delhi 110 011 br 0368 2222314 br 09436040378, 09862559914 M br 011 23795134 alma mater B.A Educated at J.N. College,Pasighat, Arunachal Pradesh occupation profession Political and Social Worker cabinet committees portfolio religion Evangelist Christian signature alt website footnotes blank1 Father s name data1 Late Shri Daying Ering blank2 Mother s name data2 Late Smt. Odam Ering blank3 data3 blank4 data4 blank5 Lok Sabha Committee Memberships data5 Military service nickname allegiance branch serviceyears rank unit commands battles awards military blank1 military data1 military blank2 military data2 military blank3 military data3 military blank4 military data4 military blank5 military data5 Ninong Ering born 3 January 1959 is an India n politician, belonging to the Indian National Congress . In the Indian general election, 2009 2009 elections , he was elected to the Lok Sabha , the lower house of the Parliament of India , from the Arunachal East Lok Sabha constituency Arunachal East constituency in Arunachal Pradesh . ref http eciresults.nic.in frmWinnerList.aspx Election Commission of India General Elections 2009 Results ref Political career Ering was a member of the A ... more details
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- Staining
, stains cell wall s purple. Crystal violet is an important component in Gram staining. DAPI DAPI ... when bound to DNA . DAPI binds with A T rich repeats of chromosomes. DAPI is also not visible with regular transmission microscopy. It may be used in living or fixed cells. DAPI stained cells are especially appropriate for cell counting. ref Levenfus, I. An efficient method for counting DAPI stained ... more details
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- Fluorescence microscope
examples of these are nucleic acid stains like DAPI and Hoechst stain Hoechst which bind the minor ... FluorescentCells.jpg Endothelial cells under the microscope. Nuclei are stained blue with DAPI, microtubules ... nucleus stained with DAPI with chromosome 13 green and 21 red centromere probes hybridized Fluorescent ... more details
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- Fluorophore
thumb Bovine Pulmonary Artery Endothelial cell nuclei stained blue with DAPI , mitochondria stained ... border 1 Probe Ex nm Em nm MW Notes Hoechst stain Hoechst 33342 343 483 616 AT selective DAPI 345 ... more details
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- Township (Republic of China)
Gukeng Dapi, Yunlin Dapi Citong, Yunlin Citong Linnei, Yunlin Linnei Erlun, Yunlin Erlun ... more details
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- Interphase
hatnote For the computer game, see Interphase video game . Image Interphase.png thumb right 159px An illustration of interphase in Gray s Anatomy . The chromatin has not yet condensed, and the cell is undergoing its normal functions. Image Interphase and part of Telophase of HT1080.PNG thumb right 159px An image of the nucleus of a cell HT1080 currently in interphase probably G1 . Note Cytoplasm of this cell or the neighboring cell is not visible top left , which is currently in the telophase of mitosis . Image taken using an optical microscope and DAPI staining of DNA. Interphase is the phase of the cell cycle in which the cell spends the majority of its time and performs the majority of its purposes including preparation for cell division . In preparation for cell division, it increases its size and makes a copy of its DNA . Interphase is also considered to be the living phase of the cell, in which the cell obtains nutrients, grows, reads its DNA, and conducts other normal cell functions. The majority of eukaryotic cells spend most of their time in interphase. Interphase does not describe a cell that is merely resting but is rather an active preparation for cell division. A common misconception is that interphase is the first stage of mitosis . However, since mitosis is the division of the Cell nucleus nucleus , prophase is actually the first stage. ref http www.biology.arizona.edu Cell bio tutorials cell cycle cells3.html The Cell Cycle & Mitosis Tutorial The Biology Project Cell Biology. University of Arizona. ref In interphase, the cell gets itself ready for mitosis or meiosis . Somatic cell s, or normal diploid cells of the body, go through mitosis in order to reproduce themselves through cell division, whereas diploid germ cell s i.e., primary spermatocyte s and primary oocyte s go through meiosis in order to create haploid gametes i.e., sperm and ovum ova for the purpose of sexual reproduction. Chromosomes are copied. Identification Under a microscope , Inte ... more details
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