Infobox protein family Symbol Apyrase Name Apyrase image PDB 1s18 EBI.jpg width caption structure and protein design of human apyrase Pfam PF06079 Pfam clan InterPro IPR009283 SMART PROSITE MEROPS SCOP 1s1d TCDB OPM family OPM protein CAZy CDD Apyrase adenosine diphosphatase is a calcium activated plasma membrane bound enzyme magnesium can also activate it EC number 3.6.1.5 that catalyst catalyses the hydrolysis of Adenosine triphosphate ATP to yield Adenosine monophosphate AMP and inorganic phosphate . Two isoenzymes are found in commercial preparations from S. tuberosum . One with a higher ratio of substrate selectivity for ATP ADP approx 10 and another with no selectivity ratio 1 . It can also act on Adenosine diphosphate ADP and other nucleoside triphosphates and diphosphates with the general reaction being NTP NDP Pi NMP 2Pi. The salivary apyrases of blood feeding arthropod s are nucleotide hydrolysing enzyme s are implicated in the inhibition of host platelet aggregation through the hydrolysis of extracellular adenosine diphosphate . ref name pmid12234496 cite journal author Smith TM, Hicks Berger CA, Kim S, Kirley TL title Cloning, expression, and characterization of a soluble calcium activated nucleotidase, a human enzyme belonging to a new family of extracellular nucleotidases journal Arch. Biochem. Biophys. volume 406 issue 1 pages 105 15 year 2002 month October pmid 12234496 doi 10.1016 S0003 9861 02 00420 4 url ref References reflist External links MeshName Apyrase InterPro content IPR009283 Category Protein families Category EC 3.6.1 hydrolase stub Acid anhydride hydrolases pt Apirase ... more details
of residues in the apyrase conserved regions. journal Biochemistry volume 40 issue 13 pages ... and conversion of an ecto apyrase to an ecto NTPase. journal Biochemistry volume 38 issue 18 pages ... Cloning, sequencing, and expression of a human brain ecto apyrase related to both the ecto ATPases ... more details
enzyme Name exopolyphosphatase EC number 3.6.1.11 CAS number 9024 85 5 IUBMB EC number 3 6 1 11 GO code 0004309 image width caption In enzymology , an exopolyphosphatase EC number 3.6.1.11 is an enzyme that catalysis catalyzes the chemical reaction polyphosphate n H sub 2 sub O math rightleftharpoons math polyphosphate n 1 phosphate Thus, the two substrate biochemistry substrates of this enzyme are polyphosphate n and water H sub 2 sub O , whereas its two product chemistry products are polyphosphate n 1 and phosphate . This enzyme belongs to the family of hydrolase s, specifically those acting on acid anhydrides in phosphorus containing anhydrides. The systematic name of this enzyme class is polyphosphate phosphohydrolase . Other names in common use include metaphosphatase , acid phosphoanhydride phosphohydrolase , and Gra Pase . This enzyme participates in purine metabolism . Structural studies As of late 2007, 4 tertiary structure structures have been solved for this class of enzymes, with Protein Data Bank PDB accession codes PDB link 1T6C , PDB link 1T6D , PDB link 1U6Z , and PDB link 2FLO . References reflist 1 cite journal author Grossman D and Lang K date 1962 title Anorganische Poly und Metaphosphatasen sowie Polyphosphate im tierischen Zellkern journal Biochem. Z. volume 336 pages 351&ndash 370 cite journal author Krishman PS date 1952 title Apyrase, pyrophosphatase and metaphosphatase of Penicillium chrysogenum journal Arch. Biochem. Biophys. volume 37 pages 224&ndash 234 doi 10.1016 0003 9861 52 90180 X pmid 14953432 issue 1 cite journal author Malmgren H date 1952 title Enzymatic behavior of polymetaphosphate. V. Purification and specificity of the enzyme journal Acta Chem. Scand. volume 6 pages 16&ndash 26 doi 10.3891 acta.chem.scand.06 0016 last2 Malmgren first2 H. last3 kerstr m first3 . last4 Finsnes first4 Ernst last5 S rensen first5 J Rgine Stene last6 S rensen first6 Nils Andreas hydrolase stub Category EC 3.6.1 Category Enzymes of known structu ... more details
biology primer and incubated with the enzymes DNA polymerase , ATP sulfurylase , luciferase and apyrase .... Unincorporated nucleotides and ATP are degraded by the apyrase , and the reaction can restart with another ... beads and enzymatic template preparation apyrase exonuclease . Commercialization The company Pyrosequencing ... more details
Orphan date February 2009 PBB geneid 957 Ectonucleoside triphosphate diphosphohydrolase 5 is an enzyme that in humans is encoded by the ENTPD5 gene . ref name pmid9271669 cite journal author Chadwick BP, Frischauf AM title Cloning and mapping of a human and mouse gene with homology to ecto ATPase genes journal Mamm Genome volume 8 issue 9 pages 668 72 year 1997 month Oct pmid 9271669 pmc doi 10.1007 s003359900534 ref ref name pmid9676430 cite journal author Chadwick BP, Frischauf AM title The CD39 like gene family identification of three new human members CD39L2, CD39L3, and CD39L4 , their murine homologues, and a member of the gene family from Drosophila melanogaster journal Genomics volume 50 issue 3 pages 357 67 year 1998 month Oct pmid 9676430 pmc doi 10.1006 geno.1998.5317 ref ref name entrez cite web title Entrez Gene ENTPD5 ectonucleoside triphosphate diphosphohydrolase 5 url http www.ncbi.nlm.nih.gov sites entrez?Db gene&Cmd ShowDetailView&TermToSearch 957 accessdate ref The PBB Summary template is automatically maintained by Protein Box Bot. See Template PBB Controls to Stop updates. PBB Summary section title summary text ENTPD5 is similar to E type nucleotidases NTPases ecto ATPase apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase conserved regions which is characteristic of NTPases. ref name entrez References reflist Further reading refbegin 2 PBB Further reading citations cite journal author Mulero JJ, Yeung G, Nelken ST, Ford JE title CD39 L4 is a secreted human apyrase, specific for the hydrolysis of nucleoside diphosphates journal J. Biol. Chem. volume 274 issue 29 pages 20064 7 year 1999 pmid 10400613 doi 10.1074 jbc.274.29.20064 cite journal author Recio JA title The human PCPH proto oncogene cDNA identification, primary structure, chromosomal mapping, and expression in normal and tumor cells journal Mol. Carcinog. volume 27 issue 3 pages 229 36 year 2000 pmid 10708485 doi 10.1002 SICI 1098 2 ... more details
Orphan date February 2009 PBB geneid 955 Ectonucleoside triphosphate diphosphohydrolase 6 is an enzyme that in humans is encoded by the ENTPD6 gene . ref name pmid9676430 cite journal author Chadwick BP, Frischauf AM title The CD39 like gene family identification of three new human members CD39L2, CD39L3, and CD39L4 , their murine homologues, and a member of the gene family from Drosophila melanogaster journal Genomics volume 50 issue 3 pages 357 67 year 1998 month Oct pmid 9676430 pmc doi 10.1006 geno.1998.5317 ref ref name entrez cite web title Entrez Gene ENTPD6 ectonucleoside triphosphate diphosphohydrolase 6 putative function url http www.ncbi.nlm.nih.gov sites entrez?Db gene&Cmd ShowDetailView&TermToSearch 955 accessdate ref The PBB Summary template is automatically maintained by Protein Box Bot. See Template PBB Controls to Stop updates. PBB Summary section title summary text ENTPD6 is similar to E type nucleotidases NTPases . NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase conserved regions which is characteristic of NTPases. ref name entrez References reflist Further reading refbegin 2 PBB Further reading citations cite journal author Chadwick BP, Frischauf AM title Cloning and mapping of a human and mouse gene with homology to ecto ATPase genes. journal Mamm. Genome volume 8 issue 9 pages 668 72 year 1997 pmid 9271669 doi 10.1007 s003359900534 cite journal author Hicks Berger CA, Chadwick BP, Frischauf AM, Kirley TL title Expression and characterization of soluble and membrane bound human nucleoside triphosphate diphosphohydrolase 6 CD39L2 . journal J. Biol. Chem. volume 275 issue 44 pages 34041 5 year 2000 pmid 10948193 doi 10.1074 jbc.M004723200 cite journal author Yeung G, Mulero JJ, McGowan DW, et al. title CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heart. journal Biochemistry volume 39 issue 42 pages 12916 23 year 2000 pmid 11041856 doi 10.1021 bi000959z ci ... more details
PBB geneid 953 Ectonucleoside triphosphate diphosphohydrolase 1 ENTPD1 also known as CD39 C luster of D ifferentiation 39 , is a human gene . ref cite web title Entrez Gene ENTPD1 Ectonucleoside triphosphate diphosphohydrolase 1 url http www.ncbi.nlm.nih.gov sites entrez?Db gene&Cmd ShowDetailView&TermToSearch 953 accessdate ref The PBB Summary template is automatically maintained by Protein Box Bot. See Template PBB Controls to Stop updates. PBB Summary section title summary text References reflist See also Cluster of differentiation Further reading refbegin 2 PBB Further reading citations cite journal author Maliszewski CR title The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization journal J. Immunol. volume 153 issue 8 pages 3574 83 year 1994 pmid 7930580 doi author separator , author2 Delespesse GJ author3 Schoenborn MA display authors 3 last4 Armitage first4 RJ last5 Fanslow first5 WC last6 Nakajima first6 T last7 Baker first7 E last8 Sutherland first8 GR last9 Poindexter first9 K cite journal author Christoforidis S title Purification and properties of human placental ATP diphosphohydrolase journal Eur. J. Biochem. volume 234 issue 1 pages 66 74 year 1996 pmid 8529670 doi 10.1111 j.1432 1033.1995.066 c.x author separator , author2 Papamarcaki T author3 Galaris D display authors 3 last4 Kellner first4 Roland last5 Tsolas first5 Orestes cite journal author Wang TF, Guidotti G title CD39 is an ecto Ca2 ,Mg2 apyrase journal J. Biol. Chem. volume 271 issue 17 pages 9898 901 year 1996 pmid 8626624 doi 10.1074 jbc.271.17.9898 cite journal author Kaczmarek E title Identification and characterization of CD39 vascular ATP diphosphohydrolase journal J. Biol. Chem. volume 271 issue 51 pages 33116 22 year 1997 pmid 8955160 doi 10.1074 jbc.271.51.33116 author separator , author2 Koziak K author3 S vigny J display authors 3 last4 Siegel first4 JB last5 Anrather first5 J last6 Beaudoin first6 AR last7 Bach first7 FH last8 Robson first8 S ... more details
This list contains a list of Enzyme Commission number EC numbers for the third group, EC 3 , hydrolases , placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology . EC 3.1 Acting on Ester Bonds EC 3.1.1 Carboxylic Ester Hydrolases EC number 3.1.1.1 carboxylesterase EC number 3.1.1.2 arylesterase EC number 3.1.1.3 triacylglycerol lipase EC number 3.1.1.4 phospholipase A2 EC number 3.1.1.5 lysophospholipase EC number 3.1.1.6 acetylesterase EC number 3.1.1.7 acetylcholinesterase EC number 3.1.1.8 cholinesterase EC number 3.1.1.9 deleted EC number 3.1.1.10 tropinesterase EC number 3.1.1.11 pectinesterase EC number 3.1.1.12 deleted EC number 3.1.1.13 sterol esterase EC number 3.1.1.14 chlorophyllase EC number 3.1.1.15 L arabinonolactonase EC number 3.1.1.16 deleted, mixture of EC 5.3.3.4 and EC 3.1.1.24 EC number 3.1.1.17 gluconolactonase EC number 3.1.1.18 deleted, included in EC 3.1.1.17 EC number 3.1.1.19 uronolactonase EC number 3.1.1.20 tannase EC number 3.1.1.21 retinyl palmitate esterase EC number 3.1.1.22 hydroxybutyrate dimer hydrolase EC number 3.1.1.23 acylglycerol lipase EC number 3.1.1.24 3 oxoadipate enol lactonase EC number 3.1.1.25 1,4 lactonase EC number 3.1.1.26 galactolipase EC number 3.1.1.27 4 pyridoxolactonase EC number 3.1.1.28 acylcarnitine hydrolase EC number 3.1.1.29 aminoacyl tRNA hydrolase EC number 3.1.1.30 D arabinonolactonase EC number 3.1.1.31 6 phosphogluconolactonase EC number 3.1.1.32 phospholipase A1 EC number 3.1.1.33 6 acetylglucose deacetylase EC number 3.1.1.34 lipoprotein lipase EC number 3.1.1.35 dihydrocoumarin hydrolase EC number 3.1.1.36 limonin D ring lactonase EC number 3.1.1.37 steroid lactonase EC number 3.1.1.38 triacetate lactonase EC number 3.1.1.39 actinomycin lactonase EC number 3.1.1.40 orsellinate depside hydrolase EC number 3.1.1.41 cephalosporin C deacetylase EC number 3.1.1.42 chlorogenate hydrolase EC number 3.1.1.43 a amino acid ester ... more details
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